![High-throughput primer design by scoring in piecewise logistic model for multiple polymerase chain reaction variants | Scientific Reports High-throughput primer design by scoring in piecewise logistic model for multiple polymerase chain reaction variants | Scientific Reports](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fs41598-022-25561-z/MediaObjects/41598_2022_25561_Fig1_HTML.png)
High-throughput primer design by scoring in piecewise logistic model for multiple polymerase chain reaction variants | Scientific Reports
![Formation of primer-dimer through complementarity between the 3' ends... | Download Scientific Diagram Formation of primer-dimer through complementarity between the 3' ends... | Download Scientific Diagram](https://www.researchgate.net/publication/258265847/figure/fig1/AS:392786089136128@1470658909194/Formation-of-primer-dimer-through-complementarity-between-the-3-ends-of-two-primers-A.png)
Formation of primer-dimer through complementarity between the 3' ends... | Download Scientific Diagram
![Algorithms for primer self-complementarity (primerSelfcom) and cross... | Download Scientific Diagram Algorithms for primer self-complementarity (primerSelfcom) and cross... | Download Scientific Diagram](https://www.researchgate.net/publication/51734134/figure/fig1/AS:214000215302162@1428033035958/Algorithms-for-primer-self-complementarity-primerSelfcom-and-cross-hybridization.png)
Algorithms for primer self-complementarity (primerSelfcom) and cross... | Download Scientific Diagram
![SOLVED: Typically, primers: - Have a high (>75%) G/C content - Should not have self-complementarity - Are approximately 50 bases in length - Optimally anneal around 15-25°C - All of the above SOLVED: Typically, primers: - Have a high (>75%) G/C content - Should not have self-complementarity - Are approximately 50 bases in length - Optimally anneal around 15-25°C - All of the above](https://cdn.numerade.com/ask_images/828a012cf6084c3f8cec14c1c63dc68d.jpg)